Protocols



PRF protocols

With the Bio-PRF technology wast improvements has been made to Platelet Rich Fibrin products. It enables clinicians to create a variety of PRF products pushing the limits to cell content and resorption profile. Below please find an overview of PRF protocols.

Bio-PRF | Solid PRF membrane

The Solid PRF membrane is made using the Bio-PRF horizontal centrifuge and Solid PRF tubes (red cap). The spin time is 8 minutes and a clot is formed. This is transferred to the PRF box and dehydrated for 2-5 minutes and is then ready for use.

Compared to fixed-angle centrifugation, the cell distribution is much more evenly spread throughout the membrane and the amount of leucocytes and platelets are up to 4 times higher.

The science behind: Article on Horizontal centrifugation
Work schedule: Phlebotomy 2 minutes | centrifugation 8 minutes  |  dehydration in PRF box 2-5 minutes  |  ready for use
Materials needed: Bio-PRF centrifuge, Solid PRF tubes
Resorption profile: 2-3 Weeks

Bio-PRF | C-PRF (Concentrated PRF) 

C-PRF is made using Liquid PRF tubes (blue cap) spun in the Bio-PRF horizontal centrifuge. The spin time is 5 minutes and the Liquid PRF are created and can last in the tubes undisturbed for 30-45 minutes. The C-PRF consist of a 0,3-0,5cc layer of cells in the buffycoat zone. This layer contains a 10 fold increase in cells when compared to conventional protocols to produce injectable PRF using fixed angulation centrifuges.

The layer is extracted into a syringe and can be used right away for injection purposes or as part of a bio-bone or bio-graft procedure. Injection can be performed with as high as 32 gauge needles.

The science behind: Article on Horizontal centrifugation and article on C-PRF
Work schedule: Phlebotomy 2 minutes | centrifugation 5 minutes  |  extraction of C-PRF from Liquid tubes 1 minute   |  ready for use
Materials needed: Bio-PRF centrifuge, Liquid PRF tubes
Resorption profile: 2-3 Weeks

Bio-PRF | Bio-graft, PRF membranes or sticky bone graft, regular resorption

The sticky bone graft combines Solid PRF membranes cut into small fragments and mixed with C-PRF using Liquid PRF tubes (blue cap). It´s made using the Bio-PRF horizontal centrifuge with a combination of Solid PRF tubes and Liquid PRF tubes. The spin time is 8 minutes and a clot is formed in the Solid PRF tubes. This is transferred to the Bio-PRF box and dehydrated for 2-5 minutes and is then ready for use. The Liquid tubes has a Platelet Poor Plasma fraction in the upper 3-4 cc and a high loaded cell layer in the buffycoat area. The latter is drawn into a syringe. The PRF membrane is cut into small fragments and mixed with the bone grafting particles and the Liquid PRF is then added and the sticky bone graft is shaped. Can be used immediately.

Compared to fixed-angle centrifugation, Sticky bone grafts have a 4 times higher amount of leucocytes and platelets in their solid PRF fragments and up to a 10 fold increase in cells when compared to injectable PRF from fixed angulation centrifuges.

The science behind: Article on Horizontal centrifugation and article on C-PRF
Work schedule: Phlebotomy 2 minutes | centrifugation 8 minutes  |  dehydration in PRF box 2-5 minutes  |  extraction of C-PRF from Liquid tubes 1 minute  |  creating of fragments and mix of bone granulates minutes  |  bone graft shaping 2 minutes  |  ready for use
Materials needed: Bio-PRF centrifuge, Solid PRF tubes and Liquid PRF tubes and Allografts (preferred)
Resorption profile: 2-6 Weeks

Bio-PRF | Alb-PRF (Albumin gel PRF) membrane

Alb-PRF is made using Liquid PRF tubes (blue cap) spun using the Bio-PRF horizontal centrifuge for 8 minutes and afterwards undergoing a bio-heating process using the Bio-Heat technology.

From each Liquid PRF tube, the upper 3 cc layer of Platelet Poor Plasma fraction is drawn into a 3 cc syringe. Each is placed in the Bio-Heat device for 10 minutes. After 10 minutes the syringes are placed in the Bio-Cool™ device for 3-5 minutes. Meanwhile the C-PRF layer from each Liquid PRF tube is extracted into one syringe per tube. When the Alb-PRF has cooled down, the Albumin gel is mixed with the concentrated C-PRF layer in syringes connected together via a specialized connector and mixed.

The result is the Bio-Filler  – a gel like texture with extended resorption properties (e-PRF). To form a membrane, the Bio-Filler is sprayed onto a metal tray or the Bio-Heat tray and the membrane is shaped – see video here. 5 minutes is required to obtain the membrane structure and can be used thereafter.

The science behind: Article on Horizontal centrifugation and article on C-PRF and article on Alb-PRF
Work schedule: Phlebotomy 2 minutes | centrifugation 8 minutes  |  extraction af PPP layer from Liquid tubes 1 minute  |  Bio-Heat 10 minutes  |  Bio-Cool 3-5 minutes  |  mix 1 minute  |  shape membrane 2-4 minutes  |  rest 5 minutes  |  ready for use
Materials needed: Bio-PRF centrifuge, Liquid PRF tubes, Bio-Heat and Bio-Cool.
Resorption profile:  4-6 months

Bio-Heat | Bio-Bone, Next generation sticky bone graft, extended resorption

The Bio-Bone is a custom made bone graft with extended resorption properties and handling. Bio-Bone combines the Alb-PRF, C-PRF and bone grafting particles to form a unique bone grafting material complex. The Alb-PRF is made using Liquid PRF tubes (blue cap) spun using the Bio-PRF horizontal centrifuge for 8 minutes and afterwards undergoes Bio-heat processing.

The upper 3 cc layer of Platelet Poor Plasma fraction is drawn into a syringe. This is placed in the Bio-Heat device for 10 minutes. After 10 minutes, the syringes are placed in the Bio-Cool device for 3-5 minutes. When the Alb-PRF has cooled down, the Albumin gel is mixed with the concentrated C-PRF layer in syringes connected together via a specialized connector and mixed.

The result is an extended-PRF (e-PRF) composition which may then be displaced into one of the pre-fabricated shapes available in the Bio-Heat tray. Thereafter, bone grafting particles may then be placed overtop the e-PRF layer followed by incorporation with liquid C-PRF to produce a final Bio-Bone matrix with an exterior e-PRF membrane lasting 4-6 months. Hereafter is ready for use.

Compared to fixed-angle centrifugation, Bio-Bone matrix have a 4 times higher amount of leucocytes and platelets in their solid PRF fragments and up to a 10 fold increase in cells when compared to injectable PRF from fixed angulation centrifuges.

The science behind: Article on Horizontal centrifugation and article on C-PRF and article on Alb-PRF
Work schedule: Phlebotomy 2 minutes | centrifugation 8 minutes  |  extraction af PPP layer from Liquid tubes 1 minute  |  Bio-Heat 10 minutes  |  Bio-Cool 3-5 minutes  |  mix 1 minute  |  shape bone graft shaping 2-4 minutes  |  ready for use
Materials needed: Bio-PRF centrifuge, Liquid PRF tubes, Bio-Heat and Bio-Cooler and Allografts (preferred)
Resorption profile:  4-6 months

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